The rK39 dipstick used for ITs is the product of a gene cloned from the Leishmania genus containing a 39-amino acid repeat conserved among viscerotropic Leishmania species ( 7). The procedure of IT-rK39 takes only 10-15 minutes and requires only 10-20 µL of the peripheral blood. However, IFI requires a fluorescence microscope and is time consuming. The serological tests IFI and IT-rK39 have the advantage of being minimally invasive and they can be performed in large numbers ( 6). VL can be life-threatening, and because 90% cases of VL occur in Brazil, reliable and rapid diagnosis of VL is required ( 4).Īs stated by the MS, VL case confirmation is based on clinical suspicion and positive laboratory diagnosis via either parasitological tests (PTs), which are dependent on invasive procedures such as bone marrow aspiration or biopsy, or serological tests such as indirect immunofluorescence (IFI) or immunochromatographic tests (ITs) using rK39 recombinant antigens ( 5).
Leishmania infantum chagasi is responsible for VL in the New World, with typical clinical signs and symptoms of splenomegaly, hepatomegaly, and fever ( 3). VL is the most severe form of leishmaniasis and it causes high morbidity and mortality in developing countries ( 1, 2). These tests are mainly distributed by the Ministério da Saúde do Brazil-MS (Ministry of Health) only to the Laboratórios Centrais de Saúde Publica - LACENs (Central Public Health Laboratories) and are predominantly provided to public hospitals. Being listed here should not suggest sponsorship.At the time of the widespread availability of rapid diagnostic tests for SARS-CoV-2-the causative virus of the COVID-19 pandemic-from drugstores throughout Brazil, there is a distinct lack of use of rapid diagnostic tests for visceral leishmaniasis (VL) at the bedsides of hospitalized patients. * With the exception of our own, these servers are not maintained by Wowhead or Fanbyte and we're not responsible for any activities therein. Photonic Playground (emphasis in screenshots) Lord of the Reins (Mount Collecting Community) Jaina's Buddies - hub for players that want to get the Glacial Tidestorm mount. Shaman: Earthshrine & Ancestral Guidance (Resto)
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